Consequently, we transfected BIRC2-overexpression plasmids into HCC cells with NAP1L1 knockdown and verified the apoptosis level, cell morphology, mitochondrial membrane potential and expression of apoptotic proteins via flow cytometry, transmission electron microscopy, laser confocal microscopy and western blotting, respectively. The gene discussed is BIRC2; the disease is hepatocellular carcinoma.