Consequently, we transfected BIRC2-overexpression plasmids into HCC cells with NAP1L1 knockdown and verified the apoptosis level, cell morphology, mitochondrial membrane potential and expression of apoptotic proteins via flow cytometry, transmission electron microscopy, laser confocal microscopy and western blotting, respectively. This evidence concerns the gene NAP1L1 and hepatocellular carcinoma.