To examine the effects of BIRC2 on the proliferation and apoptosis of HCC cells, we transfected the cells with BIRC2-overexpression plasmids and siRNAs targeting BIRC2 and verified the transfection efficiency via qRT-PCR and WB (Supplementary Fig. 4A–H). The gene discussed is BIRC2; the disease is hepatocellular carcinoma.