BEAS-2B, A549, and LC-SCs (CD117 + /CD133 +) exhibited adhesiveproperties and epithelial morphology in monolayer culture conditions(Fig. 1A, B, and C).CD133 + /CD117 + LC-SCs isolated from A549 cells were also cultured as spheroidcultures to confirm cancer stem cell properties. The gene discussed is KIT; the disease is cancer.