It is deduced that NTU 101 may not regulate the balance between Th1 cells and Th2 cells but increase the FOXP3 expression of the CD4+/CD25+ ratio to promote the maturation of Treg cells, reduce the SCORE, inflammatory cell infiltration, thickening of the epidermal cell layer and dermis layer at the stimulated site, and relieve the symptoms of allergic reactions in AD. The gene discussed is CD4; the disease is allergic disease.