TSC2 and tuberous sclerosis: While models using human stem cell technology are usually two-dimensional or include the dissociation of differentiated cardiomyocytes as a mandatory intermediate step, we hypothesize that our 3D I-Wire TSC model, which is constructed from directly differentiated CMs from hiPSCs, could recapitulate aspects of human cardiac TSC pathology more closely than other models, reproduce the functional abnormalities in contractility caused by TSC2 deficiency, and more carefully replicate changes in the mechano-elastic properties and gene expression in response to treatment with rapamycin.