To characterize the kinetic effects of 2 U/kg i.t. bleomycin on various biochemical markers of lung fibrosis, we measured the concentrations of TGFβ1, IL6, TNFα, IL1β, CINC1, WISP1, VEGF, and TIMP1 in BALF at days 3, 7, and 14 following instillation. The gene discussed is TNF; the disease is pulmonary fibrosis.