Plasmids expressing short hairpin RNA (shRNA) against Ythdf1 (shY1, downregulating Ythdf1 expression) and liposome-modified tumor cells membrane (CM) would be packaged and squeezed through a polymeric membrane in an extruder to form shY1-CM nanoparticles as a Ythdf1-targeted biomimetic nanovaccine. Here, YTHDF1 is linked to neoplasm.