In 2017, Shao et al. demonstrated that chemotherapy can cleave GSDME through the cleavage of activated Caspase-3, producing GSDME-N fragments in cancer cells to induce pyroptosis.13 In CRC, the chemotherapeutic drug lobaplatin and small molecule protein Apoptin could induce GSDME-mediated pyroptosis in CRC cells.16,26 These studies suggested that inhibition of pyroptosis-related gene GSDME splicing in tumors may trigger chemoresistance in tumors. Here, CASP3 is linked to cancer.