To explore the functional significance of NURR1 in the regulation of stemness in prostate cancer cells, we evaluated the anchorage-independent growth capacities of prostate cancer cells as mediated by either genetic (NURR1 overexpression or its reduction by gene knockdown or knockout) or signal activity modifications (enhancement of NURR1 activity by C-DIM-12 or suppression of Wnt production by IWP-2) by agar-based non-adherent 3D-culture assay. The gene discussed is NR4A2; the disease is prostate carcinoma.