Immunoblot analyses validated that NURR1 overexpression or its transactivation stimulation by C-DIM-12 could induce increased expressions of multiple PCSC-associated markers (including CD44, EpCAM, Oct4a, Nanog except CD24 which is a negative PCSC marker), whereas its reduced expression or IWP-2-induced Wnt signal suppression could reduce these PCSC-associated markers in prostate cancer cells (Fig. 4E, F). This evidence concerns the gene NR4A2 and Familial prostate cancer.