Briefly, we digested in vitro cultured MICFs (cardiac fibroblasts isolated from adult mice with myocardial infarction) infected with lentiviruses tetracycline-inducible expressing MGT (polycistronic construct including Mef2c, Gata4, and Tbx5), Myo + S (separates virus driving expression of Myocd and Sall4) (Zhao et al., 2021), and eGFP, respectively, and transplanted them into hearts of mice with surgically induced MI (MI hearts hereafter). Here, TBX5 is linked to myocardial infarction.