Using gene pathway analysis (Kyoto Encyclopedia of Genes and Genomes [KEGG]/Reactome) (Figure 7, C–E) based on spatial transcriptomes, we found Ubl conjugation, TGF-β receptor activity, and SMAD binding pathways pertaining to CD73 PTM modulation were particularly enriched in OTUD4lo compared with OTUD4hi tumor areas, further supporting potential protein interactions between OTUD4 and CD73 in tumor cells. The gene discussed is OTUD4; the disease is neoplasm.