OTUD4 and colorectal cancer: To further validate the generality of the OTUD4/CD73 interaction, a series of immunoprecipitation assays across diverse cancer cell lines, including the nonsmall cell lung cancer cell HCC827, human ovarian cancer cell SKOV3, and human colorectal cancer cell HCT116, consistently showed endogenous interaction between CD73 and OTUD4 (Supplemental Figure 4, A–D).