In conclusion, TY, SC-5, TS could regulate DSS-induced colitis in mice by associated with normal intestinal microbiota balance, thereby reducing the expression levels of pro-inflammatory factors IL-6 and IL-1β, TNF-α, correlated with the activation of NF-κB and MAPK signaling pathway, and maintaining the expression of intestinal mucosal epithelial tight junction proteins. Here, TNF is linked to colitis.