rs822336 mapped to the promoter/enhancer region of PD-L1. In order to study the mechanisms underlying the modulation by rs822336 allele-specificity of the in vitro activity of anti-PD-1 associated to a differential induction of PD-L1 expression on NSCLC cells we investigated whether differential TFs binding to rs822336 allele-specificity might affect PD-L1 expression. The gene discussed is CD274; the disease is non-small cell lung carcinoma.