Besides EGFR, H460, A549, H1299, H1703 and H1437 did not carry any alterations in ALK, ROS1, BRAF V600, NTRK1/2, RET, MET and HER2. EGFRmut cancer cell lines were used as cancer cells expected to be resistant to anti-PD-1/PD-L1 therapy [61, 62]. Here, PDCD1 is linked to cancer.