TNF and cancer: In all cell lines co-culturing of activated HLA-matched PBMCs with cancer cells significantly (P < 0.001) decreased cancer cell viability (Fig. 5A) and increased apoptotic cancer cells (Fig. 5B), PBMC-mediated LDH (Fig. 5C), IFN-γ (Fig. 5D), TNFα (Fig. 5E) releases as well as the percentage of positive CD107a PBMCs (Fig. 5F) as compared to appropriate control.