In addition, after knocking down CPNE7, we also found changes in resistance‐related genes, such as ASCL2, SNHG11, PVT1 and ATG9B, as well as resistance‐related pathways, such as MAPK, RHOA and autophagy pathways, further demonstrating the impact of CPNE7 on chemotherapy resistance in CRC and the importance of our study. Here, ASCL2 is linked to colorectal carcinoma.