To test the impact of GPR41 and GPR43 on CD8+ T cell priming following viral infection, we infected C57BL/6 wildtype (WT) mice and mice lacking GPR41 and GPR43 (Ffar2–/–;Ffar3–/–) epicutaneously with HSV-1 and examined the endogenous HSV-specific CD8+ T cell response using H2-Kb-gB498-505 tetramers. Here, CD8A is linked to viral infectious disease.