The results of this study suggest that the effect of JPGS and Fer-1 alone has no significant difference in DN mice and that RSL3 abolished the therapeutic effect of JPGS, suggesting that JPGS treats DN via upregulating the expression of GPX4 pathway-related proteins, regulating iron intake, and relieving iron overload to reduce ferroptosis and improve renal injury. This evidence concerns the gene GPX4 and liver dysplastic nodule.