As for CD8+ T-cells, activation of either TLR2 or TLR5 enhances their cytotoxic activity and upregulates the expression of IFN-γ, TNF-α, and granzyme B. These two TLRs also amplify the proliferation of T-cells and exhibit a synergistic effect.392 In vitro studies also reveal that activation of TLR1/2 in tumor-specific CTLs augments their proliferative capacity and cytotoxicity.393 Notably, TLR2 on CD8 T-cells also participates in lowering the threshold for TCR signaling activation, enabling these cells to effectively generate memory cells even in response to weak TCR signals.394. This evidence concerns the gene CD8A and neoplasm.