Briefly, mouse AML cells–expressing MLL-AF9 or MLL-ENL were transduced with recombinant lentiviruses that coexpress GFP and either nontargeting control shRNAs (shNT) or mouse shRNAs that reduce FADS1 protein expression (shFads1.1 or shFads1.2) (Fig. 2A). This evidence concerns the gene KMT2A and acute myeloid leukemia.