MLLT3 and acute myeloid leukemia: Briefly, mouse AML cells–expressing MLL-AF9 or MLL-ENL were transduced with recombinant lentiviruses that coexpress GFP and either nontargeting control shRNAs (shNT) or mouse shRNAs that reduce FADS1 protein expression (shFads1.1 or shFads1.2) (Fig. 2A).