Briefly, mouse AML cells–expressing MLL-AF9 or MLL-ENL were transduced with recombinant lentiviruses that coexpress GFP and either nontargeting control shRNAs (shNT) or mouse shRNAs that reduce FADS1 protein expression (shFads1.1 or shFads1.2) (Fig. 2A). The gene discussed is FADS1; the disease is acute myeloid leukemia.