Briefly, mouse AML cells–expressing MLL-AF9 or MLL-ENL were transduced with recombinant lentiviruses that coexpress GFP and either nontargeting control shRNAs (shNT) or mouse shRNAs that reduce FADS1 protein expression (shFads1.1 or shFads1.2) (Fig. 2A). Here, MLLT1 is linked to acute myeloid leukemia.