MGMT and glioblastoma: The authors of this study examined the total numbers of methylated MGMT promoter CpG sites in 17 different locations (CpGs 1–5 are located 85 bp upstream of DMR1 and CpGs 6–17 extended 106 bp into DMR1) using MSP and CLIA-based assay and bisulfite sequencing techniques in 300 GBM patients.