In line with this, the reduction of IFN-γ, TNF-α, and IL-6 signaling (which are key cytokines that drives Th1 differentiation), and elevation of IL-10 (which is a key cytokine that drives Th2 differentiation) that observed in hemolymph- treated trypanosomiasis mice of this study, led to the ability to control parasitemia compared to other counterpart mice. This evidence concerns the gene TNF and trypanosomiasis.