CD8A and infection: Overall, our data demonstrated that while a substantial proportion of DbNP366+CD8+ and DbPA224+CD8+ TCRαβ repertoires within SFV→IAV sequentially infected mice have prototypical TCRαβ signatures of IAV-only infected mice, additional TCRαβ clonotypes are recruited after SFV→IAV infection, especially in the brain, with more extensive pairing of TRAV and TRBV.