Specifically, MCA205 fibrosarcoma tumors and K7M2 osteosarcoma tumors were treated with control solution (anti-IgG, PBS), ketotifen (10 mg/kg, i.p.), sonopermeation (MI=0.6 and NoC=32) and Doxil (3mg/kg, i.v.)+ ICI (anti-PD1, 10 mg/kg, i.p.)and their combinations (Figs. 2A, 3A). This evidence concerns the gene PDCD1 and fibrosarcoma.