These SLCs exhibited similar profiles of expression of bipotential gonad markers LHX9, GADD45G, ZFPM2; immature SC markers KRT18, INHBA/B, NCAM1 and all-stage marker GDNF. This showed that the trans-differentiation method was capable of pushing cells from DSD background genotype towards a Sertoli fate. Here, GDNF is linked to disorder of sexual differentiation.