LMNB2 and colorectal carcinoma: To achieve this in B-type lamins, we CRISPR-engineered HCT116 colorectal carcinoma epithelial cells and knocked in the mini auxin-inducible degron and mClover at the end of endogenous lamin B1 (LMNB1) and lamin B2 (LMNB2) gene loci (Fig. 1B; Additional file 1: Table S1-3) [33].