GPT and metabolic dysfunction-associated steatotic liver disease: Finally, Spahis et al. [90] demonstrated that 6 months of 2 g daily n-3 PUFA supplementation in 20 male children/adolescents with NAFLD resulted in decreased Fatty Liver Index, a non-invasive index of steatosis, ALT levels and ALT/AST ratio, indicating beneficial effect on liver steatosis.