Despite observing higher TILs scores among patients with pTCD8+CD28- high in the lymph and/or lung metastasis group (19.6 ± 3.9 vs. 16.5 ± 4.0%) and other metastasis sites group (6.7 ± 4.8 vs. 5.7 ± 1.2%), none of these differences reached statistical significance (Fig. 5a, right). Here, CD28 is linked to metastasis.