The baseline pTCD8+CD28- level exhibited a significant negative correlation with bone metastasis (coefficient = −0.189, P = 0.003), lymph node metastasis (coefficient = −0.152, P = 0.016), and the number of metastatic sites (coefficient = −0.200, P = 0.001) (Table 2). The gene discussed is CD28; the disease is metastatic malignant neoplasm in the lymph nodes.