ZEB1 and melanoma: In order to obtain a comprehensive view of endogenous ZEB1 direct target genes in a genome-wide manner, we performed chromatin immunoprecipitation coupled to deep sequencing (ChIP-seq) analyses with an anti-ZEB1 antibody, in untreated (ZEB1low) and TNFα-treated (ZEB1high) GLO cells at day 14, but also in the A375 melanoma cell line, which displays a ZEB1high NCSC-like expression pattern (MITFlow, NGFRhigh, SOX10+, SOX9−) (Fig. 2).