The examinations showed substantially reduced Vimentin+α-SMA+ fibroblasts distributed in the stromal tissues of xenografts generated from CRC stem cells (1597) and cancer cell lines with reduced expression of DKK4 (Fig. 3A‒C), suggesting that reduced expression of DKK4 in colorectal cancer cells reduces myofibroblasts in tumour stromal tissues. This evidence concerns the gene DKK4 and colorectal cancer.