To exclude the use of distinct Nras vector backbones as a factor influencing these murine model features, we generated an additional NrasG12V/PtenKO HCC model by directly mutating the pT3-NrasG12D-GFP vector in codon 12 from D to V, thus engineering a pT3-NrasG12V/PtenKO HCC mouse model. This evidence concerns the gene NRAS and hepatocellular carcinoma.