TGFB1 and hepatocellular carcinoma: Substantiating the flow cytometry analyses and further singling out the unique TME profile of these tumors (Supplementary Fig. 3e, f), NrasG12D/PtenKO HCC displayed an enrichment of macrophages, immature DCs, TGF-β signaling and Neutrophils pathway activity (Supplementary Fig. 3h).