In contrast to total CD8+ T cells, which had the highest accumulation in the 0- to 200-μm peritumoral region, the largest density of CD8+ GzmB+ T cells was observed in the 0- to 200-μm intratumoral region on D14 (Figure 5F), suggesting antitumor cytolytic activity at the tumor border.19 This evidence concerns the gene CD8A and neoplasm.