To elucidate the complexity of cellular compositions in tumors and determine whether cGAS EV therapy constrained tumor progression by promoting a cGAS-mediated antitumor immune response, we isolated CD45+ tumor-infiltrating immune cells on day 10 post-implantation of MC38 colon cancer cells and performed single-cell RNA sequencing (scRNA-seq) (Fig. 5A). Here, PTPRC is linked to colonic neoplasm.