ABCB11 and cholestasis: Injection of AAV8-Abcb11 sgRNA alone and co-injection of AAV8-Cyp2c70 and AAV8-Abcb11 sgRNA developed hepatomegaly and elevated plasma marker of cholestasis, alkaline phosphatase, while injection of AAV8-Cyp2c70 sgRNA alone had no such effect (Figure 2C, E).