To test whether the humanized BA profile better models the patient phenotype of cholestasis and progressive liver injury in Abcb11-deficient mice, we performed intraperitoneal single or simultaneous injections of AAV8-Cyp2c70 sgRNA and AAV8-Abcb11 sgRNA at 1 × 1012 genome copies into male L-Cas9Tg/Tg mice at 6 weeks of age. The gene discussed is ABCB11; the disease is cholestasis.