The necrotic lesions are similar to the injury pattern reported in previous studies in which Abcb11 KO mice were overloaded with cholic acids.25,26 Together, these results indicate that the simultaneous knockdown of hepatic Abcb11 and Cyp2c70 caused cholestasis and hepatocellular injury in mice without overloading hydrophobic BAs. Here, ABCB11 is linked to cholestasis.