Our findings of the disruption of the p53/p21 pathway through SQLE reduction are substantiated by the following observations; Overexpression of p53R273H and p53WT hindered the survival of CRC cells (HCT116 and HT29) treated with small interfering RNAs targeting SQLE (siSQLE) under conditions of anoikis resistance. The gene discussed is TP53; the disease is colorectal carcinoma.