HNF4A and colorectal carcinoma: As a result, this study suggests that quantifying SQLE in CRC tissues using machine learning‐based digital image analysis with fluorescence‐multiplex immunohistochemistry (mlDIA‐fmIHC) can aid doctors in predicting whether patients are at an increased risk of CRC or in need of therapy targeting the β‐catenin‐TCF/LEF complex.