In the present study, we analyzed a microarray dataset of MYCN‐knockout NB cells (GSE121529)22 to identify new downstream targets of MYCN and verified that knockdown the expression of MYCN in MYCN‐amplified NGP cells increased SESN1 expression and overexpress MYCN in non‐MYCN‐amplified AS cells decreased SESN1 expression. The gene discussed is MYCN; the disease is neuroblastoma.