Further examination of anlotinib’s regulatory effect on the CRC cell cycle, using Western blot analysis of SW620 and HCT116 cells for CCNE1, CCNE2, CDK2, CCNA2, CDK1, and Histone H3, showed reduced protein levels following anlotinib treatment, especially at high concentrations (Fig. S1G). The gene discussed is CCNA2; the disease is colorectal carcinoma.