Summarizing our results indicate that preparations of inanimate Lacticaseibacillus paracasei and Lactiplantibacillus plantarum selectively impair in vitro migration of the colorectal adenocarcinoma cell lines HT‐29 and Caco‐2 by attenuating Orai1 and STIM1 expression and leading to inhibition of Ca2+ influx through SOCE and, subsequent, FAK activation. The gene discussed is PTK2; the disease is colorectal adenocarcinoma.