PfSPZ-WT LS schizonts displayed invaginating cytomeres, marked by CSP staining, on 6 (Fig. EV4B) and 7 days (Fig. 6D) post infection, which preceded segregation of the LS cytoplasm and culminated in the formation of liver stage merozoites, visualized with MSP1 staining at 7 days of LS development. The gene discussed is ATAD1; the disease is infection.