To validate the snRNAseq of human limbus biopsies from cataract patients, we performed immunohistochemistry, immunocytochemistry, and cell purification for in vitro clonogenicity assays using the limbal progenitor markers SLC6A6 and ITGβ4 identified through snRNAseq (Fig. 5). The gene discussed is SLC6A6; the disease is cataract.