NR1H4 and metabolic dysfunction-associated steatotic liver disease: Furthermore, a prototypical Farnesoid X Receptor (FXR) agonist Obeticholic Acid (OCA) was applied in the hTCS cultured in a high FFA/high glucose medium, a type of lipogenic (lipo) medium shown to induce lipogenesis while not being toxic to cells, to determine the capacity of the hTCS to detect perturbation and/or modulation of key NAFLD-associated biomarkers11,12.