TLS with primary (CD21+ follicular dendritic cells [DCs]) and secondary (CD21+CD23+ follicular DCs) follicles were defined as mTLSs, using criteria previously employed for lung carcinoma, colorectal carcinoma and melanoma (Fig. 1A, Supplementary Fig. 2B, C)43–45. This evidence concerns the gene FCER2 and colorectal carcinoma.