As enhanced TFH cell differentiation in a TMBHigh mouse cancer model has been shown to induce larger TLSs and superior B-cell recruitment37,56, we next determined the density of CD4+CXCR5+PD1+FoxP3− TFH cells in HGSOC and NSCLC samples using multiplex immunofluorescence (panel 3) (Fig. 4A). Here, CXCR5 is linked to non-small cell lung carcinoma.