CD8A and neoplasm: To confirm our findings with an independent technology, we determined the density of key TLS immune cell populations, including mature DC-LAMP+ DCs, CD20+ B cells, CD8+ T cells and effector GZMB+CD8+ T cells within individual eTLSs and mTLSs, as well as non-TLS (nTLS) areas, in the entire tumor tissue of 68 patients with HGSOC (Study cohort 1) using immunofluorescence microscopy (Fig. 1A; Supplementary Fig. 2C).