In addition, we observed that intramuscular administration of the vaccine induced intratumoral effector memory T cells (TEM) CD8+ T cells in the subcutaneous TC-1 tumors, whereas it is currently unclear whether the CD8+ T cells have a phenotype of memory resident T lymphocytes (Trm) that have shown an important anti-tumor role, which is also worthy of further investigation. The gene discussed is CD8A; the disease is neoplasm.