We observed no clear correlation between whether a knockdown affected enhancement of HIV-1G89V CA infection by MX2 and whether it caused loss of MX2T151A activity against HIV-1G89V CA. Depletion of transmembrane, cytoplasmic, and nuclear basket Nups, as well as NTRs had similar effects on the antiviral activity of MX2T151A against both HIV-1WT and HIV-1G89V CA (Fig 7C and 7E). This evidence concerns the gene MX2 and infection.