The composition of intestinal microorganisms, leading to changes in the nature of mucus and increased secretion.162 For instance, Limosilactobacillus reuteri, present in probiotic formulations has been shown to enhance the intestinal barrier by increasing mucus thickness in a mouse model of colitis.164 Additionally, probiotics promote the expression and localization of TJ proteins and genes related to mucin production.162 All the studies cited above have been summarized in Suppl. The gene discussed is MUC5AC; the disease is colitis.