Our pathway analysis suggested decreased overload-induced myofiber growth in HFpEF could be associated with changes in MuSC homeostasis, given that Hedgehog (Hh) signaling is critical for myogenesis54 and linked to overload-induced myofiber growth,55 and apelin is a peptide myokine linked to sarcopenia that regulates MuSC function.56 This evidence concerns the gene APLN and sarcopenia.