To assess the role of platelet-derived PDI in AS progression, we genetically depleted PDI expression in megakaryocytes/platelets by crossing LA100 mice with PF4CrePDIflox/flox mice to generate LA100;PF4CrePDIflox/flox mice as shown in Supplemental Figure 2.23 The gene discussed is P4HB; the disease is aortic stenosis.