Portal plasma levels of glucagon (Figure 4E) were measured at ∼100 min after insulin administration and tended to be higher in the PRL-2903-treated T2D rats (239 ± 344 pg/mL) as compared to the control T2D rats (157 ± 151 pg/mL) or HFF rats (140 ± 97 pg/mL), although the differences were not statistically different and results were highly variable (p = 0.32). Here, INS is linked to type 2 diabetes mellitus.