Then, the expressions of TGF-β1, TGF-β2, IL-35 and IL-10 in the serum of B-ALL were analyzed by ELISA, which showed that TGF-β1, IL-35 and IL-10 were significantly increased in the B-ALL group (p < 0.01, Figure 2C). This evidence concerns the gene TGFB1 and precursor B-cell acute lymphoblastic leukemia.