For instance, a study employing single-cell RNA sequencing (scRNA-seq) on tissues from 48 patients with metastatic melanoma, which included 17 responders and 31 non-responders to anti-PD-1 treatment, has revealed that tumor-infiltrating CD8+T cells can be broadly classified into early activation, memory, effector, and exhausted states. The gene discussed is CD8A; the disease is neoplasm.