To verify whether the lack of LAG-3, TIM-3, or 2B4-mediated signaling in TCRED T cells prevents functional exhaustion and promotes immunological memory, the anti-tumor responses of TCRED-IRCOMP, TCRED-LAG-3KO, TCRED-TIM-3KO and TCRED-2B4KO cells were compared in a tumor rechallenge model. This evidence concerns the gene LAG3 and neoplasm.