Interestingly, the analysis of secreted pro-inflammatory cytokines and effector molecules showed that TIM-3-, LAG-3-, and 2B4-disrupted TCRED T cells produce higher amounts of IL-2, TNFα, sFasL and perforin compared to TCRED-IRCOMP T cells when tested with the two different multiple myeloma models (Figures 2E, F;Supplementary Figure 4A). The gene discussed is PRF1; the disease is plasma cell myeloma.