Here, we added a validation step by introducing a new dataset from the GEO database: GSE56815, the results of which showed significant differences in the expression of five ferroptosis-related Hub genes such as TP53, EGFR, TGFB1, SOX2, MAPK14, between the osteoporosis group and normal controls (Figs. 5A-E). The gene discussed is TGFB1; the disease is osteoporosis.