While Hvcn1 (encoding Hv1) gene expression in MG3 subpopulation is relatively low compared to other microglia/macrophages (MG1, 2, 4, and 5; 2.13-fold decrease, p < 0.0001), we found a small but significant elevation of Hvcn1 gene expression in the MG3 of cKO brains post-stroke (1.32-fold increase, p < 0.01; Additional file 1: Figure S7), which shall alkalinize pHi. The gene discussed is HVCN1; the disease is stroke disorder.