Nevertheless, as no differences were detected in pHi in either CD11b+/CD45+ or CD11c+ populations between sham-operated WT or cKO brains (Additional file 1: Figure S4a), the increased pHrodo fluorescent intensity (indicating acidified pHi) in both CL and IL hemisphere of cKO brains are stroke-dependent. The gene discussed is ITGAM; the disease is stroke disorder.